Journal: International journal of molecular sciences
Article Title: A Study on the Levels of Selected Proangiogenic Proteins in Human Tissues and Plasma in Relation to Brain Glioma.
doi: 10.3390/ijms26104802
Figure Lengend Snippet: Figure 2. Graphs of the changes of HIF-1α, ANG-2, and IL-1β concentrations in plasma samples depending on (A) gender, (B) alcohol use, (C) concomitant non-cancerous diseases, and (D) family history of cancer. Information about the medians is placed above the box plots. (E) Mann–Whitney-U test p-value results table.
Article Snippet: The following reagents were used for the construction of the biosensor and validation of the analytical method: recombinant HIF-1α protein, recombinant human ANG-2 protein, and recombinant human IL-1β protein; monoclonal mouse antibody against HIF-1α, monoclonal mouse antibody against ANG-2, and monoclonal mouse antibody against IL-1β (all from R&D Systems, Minneapolis, MN, USA); EDC (Nethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride) (SIGMA, Steinheim, Germany); 11-MUA 11-mercaptoundecanoic acid (Aldrich, Munich, Germany); NHS Nhydroxysuccinimide (Aldrich, Munich, Germany); buffered saline solution (PBS buffer) (Biomed, Lublin, Poland); absolute ethyl alcohol (POCh, Gliwice, Poland); and ethanolamine solution (SIGMA, Steinheim, Germany).
Techniques: Clinical Proteomics, MANN-WHITNEY